Cell Lines and Cell Cultures
Eurofins Discoverx InCELL Hunter HEK 293 BRD2(1,2) Bromodomain Cell Line, 2 vials. (96-0049C1)
The InCELL Hunter HEK 293 BRD2(1,2) Bromodomain Cell Line is a stable, engineered cell line for use in studying small molecule drug candidates that engage with regulators of epigenetics pathways. This cell line enables assessment of compound-engagement-based stabilization of BRD2(1,2) via detection of BRD2(1,2) levels. This product uses EFC technology. The cell line included overexpresses BRD2(1,2) target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/epigenetics.
Eurofins Discoverx PathHunter Jurkat CD47-Presenting Cell Line, 2 vials. (93-1149C19)
The PathHunter CD47 Ligand cell line is a stable, engineered cell line designed for use with SIRPα Checkpoint Signaling Cell Lines. When co-cultured, the CD47 expressed in this cell line will induce activity of SIRPα receptors, which may be assayed through SH2 domain protein recruitment. This product is a companion product designed to work with Immunoglobulin Superfamily Checkpoint Assays. The cell line included overexpresses CD47 cell surface ligand. When co-cultured with the SIRPa Checkpoint Signaling Cell Line, the CD47 ligand binds to the checkpoint receptor, inducing receptor activity. This receptor activity leads to recruitment of the relevant SH2 domain protein to the checkpoint receptor inside the receptor cell line and production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/checkpoint.
Innovative Research Inc Single Donor Human Ear Wax Swab 1
Single Donor Human Ear Wax Swab from Innovative Research is also known as cerumen. It is a waxy substance secreted in the ear canal to protect the skin of the ear canal, assist in cleaning and lubrication, and also provides protection against bacteria, fungi, insects, and water. This product is collected on sterile cotton tipped applicators and stored dry in a sterile tube. A sample is one donor across two swabs.
Eurofins Discoverx InCELL Hunter HEK 293 PIKFYVE Kinase Binding Cell Line, 2 vials. (96-0078C1)
The InCELL Hunter HEK 293 PIKFYVE Kinase Binding Cell Line is a stable, engineered cell line for use in studying small molecule drug candidates that engage with kinases. This cell line enables assessment of compound-engagement-based stabilization of PIKFYVE via detection of PIKFYVE levels. This product uses EFC technology. The cell line included overexpresses PIKFYVE target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is denatured by the heat treatment and no EFC signal is produced. When compound engages and heat-stabilizes the target, EA contained in the detection reagent produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Biochain Institute Inc Paraffin Tissue Section - Human Adult Normal: Brain: Cerebellar Peduncles, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Eurofins Discoverx ChemiScreen BLT1 Leukotriene Receptor Stable Cell Line, 2 vials. (HTS042C)
The ChemiScreen BLT1 Leukotriene Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. leukotriene) based activation of LTB4R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses LTB4R alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of LTB4R, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
ATCC Trypsin Neutralizing Solution
Trypsin Neutralizing Solution is specifically formulated (5% FBS in phosphate-buffered saline without calcium and magnesium) to rapidly inactivate the concentration of trypsin found in the Trypsin-EDTA for Primary Cells solution.
ATCC Human herpesvirus 5; Strain: Davis; Human cytomegalovirus
Human herpesvirus 5 strain Davis is propagated in MRC-5 cells (ATCC CCL-171). This strain was isolated from the liver biopsy of a 3-month-old human female with microencephaly and other symptoms resembling congenital toxoplasmosis. This product has applications in infectious disease research.
ATCC Clone M-3; Melanocyte; Mouse (Mus musculus)
Clone M-3 [Cloudman S91 melanoma] is a melanocyte cell line that was isolated from the skin of a mouse with melanoma. The Clone M-3, a melanin-producing cell line was adapted to cell culture by Y. Yasumura, A.H. Tashjian, and G. Sato from a Cloudman S91 melanoma in a (C X DBA) F1 male mouse obtained from the Jackson Memorial Laboratory, Bar Harbor, Maine. This cell line was deposited by G Sato.
Lonza Walkersville Mouse Macrophage Nucleofector Kit
The Nucleofector 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary mouse macrophages.The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Mouse Macrophages0.5 mL Supplement 230 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Eurofins Discoverx PathHunter CHO-K1NR β-Arrestin Cell Line, 2 vials. (93-0323C2)
The PathHunter MLNR cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. motilin) based activation of MLNR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MLNR and EA-tagged β-Arrestin-2. Activation of MLNR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Biochain Institute Inc Frozen Tissue Array - 37 Different Ovarian Tumors and 3 Corresponding Normal Controls, 5 slides/pk
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's human tumor frozen tissue arrays are designed for studying tumor tissue specific cellular localization of genes or proteins of interest in different human tissues. Same kind of tumors and corresponding normal tissues are mounted on positively charged slides.
Biochain Institute Inc Frozen Tissue Section - Human Adult Normal: Brain: Midbrain, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
Eurofins Discoverx Ready-to-Assay XCR1 Chemokine Receptor Frozen Cells, 2 Vials of Cells. (HTS053RTA)
The Ready-to-Assay XCR1 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. lymphotactin) based activation of XCR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses XCR1 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of XCR1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
ATCC Primary Uterine; Smooth Muscle Cells; Normal, Human
Primary uterine smooth muscle cells are elongated myocytes isolated from the uterus of a donor and can be used in female reproductive biology, drug testing, and oncology.